Journal: Oncology Reports
Article Title: Antitumor activities of anti‑CD44 monoclonal antibodies in mouse xenograft models of esophageal cancer
doi: 10.3892/or.2024.8806
Figure Lengend Snippet: Flow cytometry using 5-mG 2a and C 44 Mab-46-mG 2a . (A) Class-switched and defucosylated mouse IgG 2a mAbs, 5-mG 2a and C 44 Mab-46-mG 2a , were generated from C 44 Mab-5 and C 44 Mab-46 (mouse IgG 1 ), respectively. (B) CHO/CD44s and (C) KYSE770 cells were treated with 10–0.01 µg/ml of 5-mG 2a and C 44 Mab-46-mG 2a (red) or buffer control (black), followed by Alexa Fluor 488-conjugated anti-mouse IgG. Fluorescence data were analyzed using the SA3800 Cell Analyzer. mAb, monoclonal antibody.
Article Snippet: Fluorescence data were collected using the SA3800 Cell Analyzer (Sony Corp.) and analyzed using SA3800 software ver.
Techniques: Flow Cytometry, Generated, Control, Fluorescence